Scrape-loading dye transfer assay
WebAug 1, 2024 · The SL-DT assay can serve a broad range of in vitro pharmacological and toxicological needs, and can be potentially adapted for high throughput set-up systems … WebMar 8, 2024 · In scrape-loaded astrocytes, the dye traveled through functional gap junctions into adjacent gap junction–coupled astrocytes that avoided the scrape-loading. Astrocytes were pretreated with MHC1 (66.7 nM) or a known gap junction blocker, carbenoxolone (CBX) (100 μM), for either 3 or 24 hours at 37°C prior to the gap junction coupling assay.
Scrape-loading dye transfer assay
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WebMay 21, 2016 · Abstract. The scrape loading/dye transfer (SL/DT) technique is a simple functional assay for the simultaneous assessment of gap junctional intercellular communication (GJIC) in a large population of cells. The equipment needs are minimal … For over 35 years, biological scientists have come to rely on the research protocols … Scrape Loading/Dye Transfer Assay. Pavel Babica, Iva Sovadinová, Brad L. Upham; … WebMar 7, 2024 · Scrape-loading dye transfer in vitro. Gap junction-mediated intercellular coupling was evaluated by transfer of lucifer yellow dye in a scrape-loading assay as described previously with minor modifications . Briefly, astrocytes were seeded on 12-well culture plates and incubated at 37 °C and 5% humidified atmosphere.
WebFeb 1, 1987 · It employs scrape-loading to introduce a low molecular weight (MW) fluorescent dye, Lucifer yellow CH (MW 457.2) into cells in culture and allows the … WebJan 20, 2024 · Fluoranthene as a non-cytotoxic GJIC inhibitor assessed using the multiparametric scrape-loading-dye transfer (SL-DT) assay in rat liver progenitor WB-F344 cells (a 24-well plate format, 1-h exposure). (a) The effects were compared with the non-treated control and expressed as the fraction of control (FOC; FOC non-treated control = …
WebSep 17, 2024 · The scrape loading/dye transfer assay was employed to access lucifer Yellow molecules transfer efficiency mediated by cell-cell communication. Connexin43 (Cx43), Pannexin1 (Panx1), MAPK, and the PI3K/Akt signaling pathway proteins were examined via Western blotting. WebOne of these assays, the scrape-loading dye transfer (SLIDT) technique, is based on monitoring the transfer of the fluorescent dye Lucifer yellow from one cell into adjacent cells via functional gap junctions. The objective of our study was to evaluate and compare various approaches for quantifying results obtained with the SL/DT technique.
WebScrape Loading/Dye Transfer Assay Pavel Babica, Iva Sovadinová, and Brad L. Upham Abstract The scrape loading/dye transfer (SL/DT) technique is a simple functional assay …
WebFigure 7 Utility of the scrape loading/dye transfer assay to detect a toxin/toxicant s ability to modulate gap junctional intercellular communication. The last two images (6ngmr TPA) illustrate both the epifluorescent and phase images, respectively, of the same cells to show that the complete inhibition of GJIC was not associated with the death ... softmode shoesWebFeb 28, 2011 · An innovative adaptation of traditional, fluorescence microscopy-based scrape loading-dye transfer (SL-DT) assay is presented, which has been optimized to simultaneously evaluate GJIC, cell density and viability, which could be included into in vitro screening and assessment of pharmacologically and toxicologically relevant compounds. … softmod any wii guideWebAbstract. The scrape loading/dye transfer (SL/DT) technique is a simple functional assay for the simultaneous assessment of gap junctional intercellular communication (GJIC) in a … soft modding switch lite