WebbP. pastoris strains GS115 (his4) and SMD1168 (his4 pep4) were used for expression of the hGH protein. Cell culture, electro-competent cells, transformation and selection of recombinant clones were performed as described in the Pichia User Manual (Invitrogen, USA). Prior to transformation, recombinant pPIC3.5K vector WebbTargeted gene knockouts play an important role in the study of gene function. For the generation of knockouts in the industrially important yeast Pichia pastoris, several …
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Webb13 okt. 2024 · The resulting vector pPHT-G12 containing GFP/ HA-GSA12 behind the constitutive and glucose-inducible GAPDH promoter was linearized by cutting within the HIS4 gene (e.g., with Stu I) and used to transform by electroporation R2 ( his4 gsa12 ::Zeocin) and DMM1 ( his4 :: pDM1 (P AOX1 BFP-SKL, Zeocin)) cells. storage ottoman lift top
Expression of the α3/β1 isoform of human Na,K-ATPase in the ...
Webbtransfected into either COS-1 cells or Pichia pastoris strain SMD1163 (his4 pep4 prb1),15 respectively. For Pichia expression, the DNA coding region for WebbDownload scientific diagram Confirmation of the HIS4 deletion (A, B), PEP4 deletion (C, D) and double deletion (E-H) by PCR. Lanes S1 and S2, standard DNA marker. (a) … Webb14 nov. 2024 · protease-deficient strains, such as SMD1165 (his4, pep4, and prb1 deletions), SMD1163 (his4 and prb1 deletions) and SMD1168 (his4 and pep4 … storage ottoman for patio set