WebMar 3, 2015 · Importantly, the digestion patterns of plasmids recovered from BNN132 showed that the loxP-CEN6/ARSH4-loxP element was removed from the construct in three out of five transformants (lanes 3–5). The remaining transformants contained mixtures of recombined and unrecombined plasmids (lanes with mixtures are highlighted with a … WebIn the BY4741 haploid strain, the low-copy, CEN6/ARSH4 plasmids ranged from 2 to 5 copies per cell and the high copy 2μ plasmids with auxotrophic markers had a wide range of 14 to 34 copies per cell (Fig. 3a). Interestingly, the LEU2-containing 2μ plasmids had noticeably lower copy numbers than the other 2μ plasmids with auxotrophic markers ...

DesignerDeletionStrainsderivedfrom Saccharomyces …

Webfor maintenance and replication in yeast. The CEN6/ARSH4 sequence is a 518 bp hybrid DNA fragment that contains a yeast centromere sequence (CEN) and an autonomously … WebCC CEN6 - ARSH4. CC A genomic HincII/PstI fragment (1.002 kb) containing the TRP1 gene was CC inserted into the NdeI site and a cassette containing CEN6 and the ARS CC associated with histone 4 (ARSH4) was inserted into the AatII site of All ends were blunted. phet simulation plate tectonics

Efficient inter-species conjugative transfer of a CRISPR

Web中国微生物资源共享平台,微生物菌种保藏中心,泰斯拓生物提供菌株,atcc菌株,菌株,质粒载体,慢病毒，细菌，真菌,支原体,衣原体,dsmz,ccug,atcc,分子技术试验,相关的技术服务,咨询电话0574-87917803 WebIn the BY4741 haploid strain, the low-copy, CEN6/ARSH4 plasmids ranged from 2 to 5 copies per cell and the high copy 2μ plasmids with auxotrophic markers had a wide … http://genome-www.stanford.edu/vectordb/vector_descrip/COMPLETE/PRS314.SEQ.html phet simulation photoeffekt